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Anti-EGFR PHOSPHO Antibody
Also for EGFR (NM_005228)
|The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of threonine 693 (P-L-TP-P-S).|
|Human: Thr693; Mouse: Thr695; Rat: Thr694
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:40000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|EGFR (phospho-Thr693) antibody detects endogenous levels of EGFR only when phosphorylated at threonine 693.
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Western blot analysis of extracts from A431cells, using EGFR (phospho-Thr693) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using EGFR (phospho-Thr693) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HuvEc cells, using EGFR (phospho-Thr693) antibody.The picture on the right is treated with the synthesized peptide.