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Anti-EGFR PHOSPHO Antibody EP39IHCY
Also for EGFR (NM_005228)
|A synthetic peptide corresponding to residues surrounding Tyr1068 of human EGF receptor was used as immunogen. This antibody detects both phosphorylated and non-phosphorylated EGFR protein.|
||Tissue culture supernatant
|Mouse, Human (Does not react with: Rat)
||Lot dependent; please refer to CoA along with shipment
||ICC/IF: 1:100 - 1:250; WB: 1:1000 - 1:5000; FC: 1:55
|Does not react with Rat. Is unsuitable for IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for IHC-P or IP.
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Western blot - EGFR antibody [EP39IHCY]; Anti-EGFR antibody [EP39IHCY] at 1/5000 dilution + HeLa cell lysate at 10 ug.Secondary.goat anti-rabbit HRP at 1/1000 dilution.developed using the ECL technique.Predicted band size : 134 kDa.Observed band size : 170,180 kDa .
Immunocytochemistry/ Immunofluorescence - EGFR antibody [EP39IHCY]; TA303829 at 1/100 dilution staining EGFR in A431 cells by Immunofluorescence.