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Anti-EGFR PHOSPHO Antibody EP2255Y
Also for EGFR (NM_005228)
|A phospho specific peptide corresponding to residues surrounding threonine 678 of human EGFR was used as an immunogen. This antibody detects EGFR phosphorylated on thr678.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||ICC/IF: 1:100 - 1:250; WB: 1:20000 - 1:50000
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1|
|ERBB; ERBB1; HER1; mENA; PIG61|
|The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]. |
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - EGFR (phospho T678) antibody [EP2255Y]; All lanes : Anti-EGFR (phospho T678) antibody [EP2255Y] at 1/50000 dilution.Lane 1 : A431 cell lysate.Lane 2 : A431 cell lysate treated with EGF.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 134 kDa.Observed band size : 175 kDa .
Immunocytochemistry - EGFR (phospho T678) antibody [EP2255Y]; Immunofluorescent staining of EGFR (phospho T678) in A431 cells, using ab75986 at a 1/100 dilution.