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Anti-EGFR PHOSPHO Antibody EP2255Y
Also for EGFR (NM_005228)
|A phospho specific peptide corresponding to residues surrounding threonine 678 of human EGFR was used as an immunogen. This antibody detects EGFR phosphorylated on thr678.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||ICC/IF: 1:100 - 1:250; WB: 1:20000 - 1:50000
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt,IHC-P or IP.
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Western blot - EGFR (phospho T678) antibody [EP2255Y]; All lanes : Anti-EGFR (phospho T678) antibody [EP2255Y] at 1/50000 dilution.Lane 1 : A431 cell lysate.Lane 2 : A431 cell lysate treated with EGF.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 134 kDa.Observed band size : 175 kDa .
Immunocytochemistry - EGFR (phospho T678) antibody [EP2255Y]; Immunofluorescent staining of EGFR (phospho T678) in A431 cells, using TA303494 at a 1/100 dilution.