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Home Antibody All anti-EGFR antibodies

Anti-EGFR PHOSPHO Antibody EP2255Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303494
  • Rabbit monoclonal antibody against EGFR Phospho (pT678) (EP2255Y ) (phospho-specific)
100ul 325 3-7 Days
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WB(1)
IF(1)
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Also for EGFR (NM_005228)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding threonine 678 of human EGFR was used as an immunogen. This antibody detects EGFR phosphorylated on thr678.
Clone NameEP2255Y IsotypeTissue culture supernatant
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsICC/IF: 1:100 - 1:250; WB: 1:20000 - 1:50000
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens epidermal growth factor receptor (EGFR), transcript variant 1
Alternative NameERBB; ERBB1; HER1; mENA; PIG61
Database LinkNP_005219
FunctionThe protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq].
Related Pathway
Adherens junction
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - EGFR (phospho T678) antibody [EP2255Y]; All lanes : Anti-EGFR (phospho T678) antibody [EP2255Y] at 1/50000 dilution.Lane 1 : A431 cell lysate.Lane 2 : A431 cell lysate treated with EGF.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 134 kDa.Observed band size : 175 kDa .
IF Image
Immunocytochemistry - EGFR (phospho T678) antibody [EP2255Y]; Immunofluorescent staining of EGFR (phospho T678) in A431 cells, using TA303494 at a 1/100 dilution.

 

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