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Anti-EGFR Antibody E235
Also for EGFR (NM_201283)
|A synthetic peptide corresponding to residues in C-terminus of human EGFR was used as immunogen. The antibody does not cross-react with other ERBB family members.|
||Protein A purified
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IF, FC
||WB: 1:10000; ICC: 1:50; FC: 1:10 - 1:1000; IP: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
|Is unsuitable for IHC.
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Western blot - EGFR antibody [E235]; Anti-EGFR antibody [E235] at 1/10000 dilution + A431 cell lysate.Predicted band size : 134 kDa.Observed band size : 180 kDa .
Immunocytochemistry - Anti-EGFR antibody [E235]; Immunofluorescent staining of A431 cells using TA303490.
Flow Cytometry - Anti-EGFR antibody [E235]; Overlay histogram showing A431 cells stained with TA303490 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.