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Anti-EGFR PHOSPHO Antibody EP2259Y
Also for EGFR (NM_005228)
|A synthetic phospho-peptide corresponding to residues surrounding Serine 1070 and 1071 of human EGF receptor was used as immunogen.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:500000 - 1:1e+006; IP: 1:30; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt or IHC-P.
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Western blot - EGFR (phospho S1070 + S1071) antibody [EP2259IHCY]; All lanes : Anti-EGFR (phospho S1070 + S1071) antibody [EP2259Y] at 1/1000000 dilution.Lane 1 : A431 cell lysate.Lane 2 : A431 cell lysate treated with EGF.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 134 kDa.Observed band size : 180 kDa .
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1070 + S1071) antibody [EP2259IHCY]; Immunofluorescence staining of A431 cells using TA303489 at 1/100-1/250 dilution.