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Home Antibody All anti-EGFR antibodies

Anti-EGFR PHOSPHO Antibody EP2259Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303489
  • Rabbit monoclonal antibody against EGFR Phospho (pS1070/pS1071) (EP2259IHCY ) (phospho-specific)
100ul $325 3-7 Days
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WB(1)
IF(1)
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Also for EGFR (NM_005228)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Serine 1070 and 1071 of human EGF receptor was used as immunogen.
Clone NameEP2259Y IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:500000 - 1:1e+006; IP: 1:30; ICC/IF: 1:100 - 1:250
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt or IHC-P.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt or IHC-P.

Reference Data

Target NameHomo sapiens epidermal growth factor receptor (EGFR), transcript variant 1
Alternative NameERBB; ERBB1; HER1; mENA; PIG61
Database LinkNP_005219
FunctionThe protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq].
Related Pathway
Adherens junction
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - EGFR (phospho S1070 + S1071) antibody [EP2259IHCY]; All lanes : Anti-EGFR (phospho S1070 + S1071) antibody [EP2259Y] at 1/1000000 dilution.Lane 1 : A431 cell lysate.Lane 2 : A431 cell lysate treated with EGF.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 134 kDa.Observed band size : 180 kDa .
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1070 + S1071) antibody [EP2259IHCY]; Immunofluorescence staining of A431 cells using TA303489 at 1/100-1/250 dilution.

 

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