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Anti-EEF2 PHOSPHO Antibody EP833Y
Also for EEF2 (NM_001961)
|A synthetic phospho-peptide corresponding to residues surrounding Thr 56/58 of human eEF2 was used as immunogen. The antibody only detects eEF2 phosphorylated on Tyreonine 56/58.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens eukaryotic translation elongation factor 2 (EEF2)|
|EEF-2; EF-2; EF2; SCA26|
|The eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated, and phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4) |
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Western blot - EEF2 (phospho T56 + T58) antibody [EP833Y]; All lanes : Anti-EEF2 (phospho T56 + T58) antibody [EP833Y] at 1/2000 dilution.Lane 1 : Untreated 293 cell lysate.Lane 2 : Serum starved 293 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 95 kDa.Observed band size : 95 kDa.