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Home Antibody All anti-EEF2 antibodies

Anti-EEF2 PHOSPHO Antibody EP833Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307499
  • Rabbit monoclonal antibody against eEF2 Phospho (pT56/58) (clone EP833Y ) (Phospho-Specific)
100ul 325 3-7 Days
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WB(1)
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Also for EEF2 (NM_001961)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Thr 56/58 of human eEF2 was used as immunogen. The antibody only detects eEF2 phosphorylated on Tyreonine 56/58.
Clone NameEP833Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB Suggested DilutionsWB: 1:2000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens eukaryotic translation elongation factor 2 (EEF2)
Alternative NameEEF-2; EF-2; EF2; SCA26
Database LinkNP_001952
FunctionThe eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated, and phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4)
Related Pathway

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WB Image
Western blot - EEF2 (phospho T56 + T58) antibody [EP833Y]; All lanes : Anti-EEF2 (phospho T56 + T58) antibody [EP833Y] at 1/2000 dilution.Lane 1 : Untreated 293 cell lysate.Lane 2 : Serum starved 293 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 95 kDa.Observed band size : 95 kDa.

 

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