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Anti-DUX4 Antibody E55
Also for DUX4 (NM_033178)
|Recombinant proteins, a mixture of two different protein fragments, of human DUX4 were used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||ICC/IF: Use at an assay dependent concentration; WB: 1:1000 - 1:10000; FC: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens double homeobox 4 (DUX4)|
|The DUX4 antibody, clone E5-5, was recently described in the publication called "Facioscapulohumeral dystrophy: incomplete suppression of a retrotransposed gene." (Snider L et al. PLoS Genet. 2010 Oct 28;6(10):e1001181).|
DUX4, also known as double homeobox 4, contains two homeoboxes; the repeat-array and open reading frame (ORF) is conserved in other mammals. There was no evidence for transcription from standard cDNA libraries; however, RT-PCR and in-vitro expression experiments indicate that DUX4 has been reported to function as a transcriptional activator of paired-like homeodomain transcription factor 1. Mutations in DUX4 cause autosomal dominant facioscapulohumeral muscular dystrophy (FSHD) (1).
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Western blot - Anti-Dux4 antibody [E55]; All lanes : Anti-Dux4 antibody [E55] at 1/1000 dilution.Lane 1 : Human testis lysate.Lane 2 : HT 1080 cell lysate.Lane 3 : PC 3 cell lysate.Lane 4 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 45 kDa.
Flow Cytometry - Anti-Dux4 antibody [E55]; ab124699 at 1/10 dilution staining Dux4 in permeabilized HeLa cells by Flow cytometry (red) or a rabbit IgG as negative control (green).