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Anti-DRD1 Antibody EP1560Y
Also for DRD1 (NM_000794)
|A synthetic peptide corresponding to the C-terminal of human D(1A) dopamine receptor protein was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||FC: 1:100 - 1:1000; WB: 1:1000 - 1:10000
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens dopamine receptor D1 (DRD1)|
|D(1A) dopamine receptor (DRD1) belongs to the G-protein coupled receptor 1 family and it is one of the five types of dopamine receptors (D1-D5) (1). DRD1 is the most expressed dopamine receptor in central nervous system and it is involved in the regulation of neuronal growth & development, and modulation of dopamine receptor D2-mediated events. G protein coupled DRD1 activates adenylyl cyclase which leads to activation of cyclic AMP dependent protein kinases. Polymorphism of DRD1 gene has been implicated in bipolar I disorder and attention deficit hyperactivity disorder (ADHD) (1,2). |
Signaling by GPCR
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Western blot - Dopamine Receptor D1 antibody [EP1560Y]; Anti-Dopamine Receptor D1 antibody [EP1560Y] at 1/20000 dilution + SH-SY5Y cell lysate at 10 µg.Secondary.HRP labelled goat anti rabbit at 1/2000 dilution.Predicted band size : 49 kDa.Observed band size : 75 kDa .
Flow Cytometry - Anti-Dopamine Receptor D1 antibody [EP1560Y]; Overlay histogram showing SH-SY5Y cells stained with ab81296 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.