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Home Antibody All anti-DPH2 antibodies

Anti-DPH2 TRUEMAB Antibody Clone OTI4E7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA504852 DPH2 mouse monoclonal antibody, clone OTI4E7 (formerly 4E7) 100ul $379 In Stock
LC419990 DPH2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF504852 Carrier-free (BSA/glycerol-free) DPH2 mouse monoclonal antibody, clone OTI4E7 (formerly 4E7) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IHC(11)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human DPH2(NP_001375) produced in HEK293T cell.
Clone NameClone OTI4E7 IsotypeIgG1
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration1 mg/ml
Guaranteed Application *WB, IHC, FC Suggested Dilutions WB 1:500~2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 51.9 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens DPH2 homolog (S. cerevisiae) (DPH2), transcript variant 1
Alternative NameDPH2L2
Database LinkNP_001375
Entrez Gene 1802 Human
Entrez Gene 67728 Mouse
Entrez Gene 298452 Rat
Entrez Gene 475387 Dog
FunctionThis gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPH2 (RC201382, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPH2.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DPH2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504852)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504852)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504852)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504852)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504852)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504852)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504852)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504852)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504852)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504852)
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504852)
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HEK293T cells transfected with either RC201382 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DPH2 antibody(TA504852), and then analyzed by flow cytometry.
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