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Anti-DNTT Antibody EPR2976Y
Also for DNTT (NM_001017520)
|A synthetic peptide corresponding to residues on the C terminus of human TDT was used as an immunogen.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:10000 - 1:50000; IP: 1:20; ICC/IF: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt or IHC-P.
|Homo sapiens DNA nucleotidylexotransferase (DNTT), transcript variant 2|
Entrez Gene 1791 Human
|This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq]. |
| Non-homologous end-joiningHematopoietic cell lineage|
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Western blot - TdT antibody [EPR2976Y]; All lanes : Anti-TdT antibody [EPR2976Y] at 1/10000 dilution.Lane 1 : Human thymus lysate.Lane 2 : Jurkat lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 58 kDa.Observed band size : 58 kDa.Additional bands at : 24 kDa,35 kDa. We are unsure as to the identity of these extra bands.