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Anti-DNMT3B Antibody EPR3523
Also for DNMT3B (NM_175848)
|A synthetic peptide corresponding to residues in human Dnmt3b was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:5000; ICC/IF: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 2|
|ICF; ICF1; M.HsaIIIB|
|DNA (cytosine-5)-methyltransferase 3B (Dnmt3b) is a DNA methyltransferase that is essential for mammalian development potentially through its transcription and repression activity (1). It is one of three different families of DNA (cytosine-5) methyltransferases, Dnmt1, Dnmt2, Dnmt3 (Dnmt3a and Dnmt3b). Dnmt3a and Dnmt3b form complexes with the major maintenance enzyme Dnmt1 (2). Depletion of Dnmt3b has been shown to induce apoptosis of human cancer cells, and reactivate methylation-silenced gene expression (3). Methylation defects in ICF syndrome may also result from impaired stimulation of Dnmt3b activity (4). |
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Western blot - Anti-Dnmt3b antibody [EPR3523]; All lanes : Anti-Dnmt3b antibody [EPR3523] at 1/1000 dilution.Lane 1 : Flag-tagged Dnmt3b.Lane 2 : Dnmt3b knockout mouse embryonic stem cell extract.Lane 3 : Wild type mouse embryonic stem cell extract.Lane 4 : Wild type mouse embryonic stem cell extract.Lysates/proteins at 40 µg per lane.Secondary.Donkey anti-rabbit IgG conjugated to HRP (NA934) at 1/1000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 96 kDa.Observed band size : 110 kDa .Exposure time : 5 minutesImage courtesy of anonymous abreview
Western blot - Dnmt3b antibody [EPR3523]; All lanes : Anti-Dnmt3b antibody [EPR3523] at 1/5000 dilution.Lane 1 : A549 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : A431 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP conjugate at 1/2000 dilution.Predicted band size : 96 kDa.Observed band size : 96 kDa.