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Home Antibody All anti-DNMT3B antibodies

Anti-DNMT3B Antibody EPR3523

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA306991
  • Rabbit monoclonal antibody against Dnmt3b (clone EPR3523 )
100ul 325 In Stock
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WB(2)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Dnmt3b was used as an immunogen.
Clone NameEPR3523 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB Suggested DilutionsWB: 1:1000 - 1:5000; ICC/IF: 1:50 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 2
Alternative NameICF; ICF1; M.HsaIIIB
Database LinkNP_787044
FunctionDNA (cytosine-5)-methyltransferase 3B (Dnmt3b) is a DNA methyltransferase that is essential for mammalian development potentially through its transcription and repression activity (1). It is one of three different families of DNA (cytosine-5) methyltransferases, Dnmt1, Dnmt2, Dnmt3 (Dnmt3a and Dnmt3b). Dnmt3a and Dnmt3b form complexes with the major maintenance enzyme Dnmt1 (2). Depletion of Dnmt3b has been shown to induce apoptosis of human cancer cells, and reactivate methylation-silenced gene expression (3). Methylation defects in ICF syndrome may also result from impaired stimulation of Dnmt3b activity (4).
Related Pathway

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WB Image
Western blot - Anti-Dnmt3b antibody [EPR3523]; All lanes : Anti-Dnmt3b antibody [EPR3523] at 1/1000 dilution.Lane 1 : Flag-tagged Dnmt3b.Lane 2 : Dnmt3b knockout mouse embryonic stem cell extract.Lane 3 : Wild type mouse embryonic stem cell extract.Lane 4 : Wild type mouse embryonic stem cell extract.Lysates/proteins at 40 µg per lane.Secondary.Donkey anti-rabbit IgG conjugated to HRP (NA934) at 1/1000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 96 kDa.Observed band size : 110 kDa .Exposure time : 5 minutesImage courtesy of anonymous abreview
WB Image
Western blot - Dnmt3b antibody [EPR3523]; All lanes : Anti-Dnmt3b antibody [EPR3523] at 1/5000 dilution.Lane 1 : A549 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : A431 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP conjugate at 1/2000 dilution.Predicted band size : 96 kDa.Observed band size : 96 kDa.

 

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