Anti-Dnmt1 Antibody, EPR3522

Also for Dnmt1 (NM_001130823)

OriGene Data

Immunogen	A synthetic peptide corresponding to residues at the C-terminus of human Dnmt1 was used as an immunogen.
Clone Name	EPR3522	Isotype
Species Reactivity	Human	Concentration
Guaranteed Application *	WB, IF	Suggested Dilutions	WB: 1:1,000 - 5,000; IHC 1:100 - 250; ICC: 1:100 - 250; IP: 1:10; FC: 1:50;
Buffer	Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Purification	(Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1
Alternative Name	AIM; CXXC9; DNMT; HSN1E; MCMT
Database Link	NP_001124295
Function	Dnmt1 is one of three families of enzymes (Dnmt1, Dnmt2, Dnmt3 (a and b)) that have a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Abnormal methylation patterns are associated with certain human tumors and developmental abnormalities (1). Dnmt1 is responsible for maintenance of the DNA methylation pattern after DNA replication. Dnmt1 is a large protein and can be subdivided into an N- and a C-terminal part. The C-terminal domain harbours the catalytic center. The N-terminal part of the enzyme comprises several individual domains, and plays a role in intracellular targeting and regulation of the enzyme’s activity. Dnmt1 shows a preference for hemimethylated CG sites and the specificity of Dnmt1 for hemimethylated target sites is a key property for that function (2).
Related Pathway

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WB Image

Western blot analysis on (A) HuT-78 and (B) Jurkat and (C) 293T cell lysates using anti-Dnmt1 RabMAb.

IF Image

Immunofluorescent staining of HeLa cells using anti-Dnmt1 RabMAb.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
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DyLight® 594
DyLight® 633

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Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-Dnmt1 Antibody EPR3522

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data