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Home Antibody All anti-DNMT1 antibodies

Anti-DNMT1 Antibody EPR3521(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307586
  • Rabbit monoclonal antibody against Dnmt1(clone EPR3521(2))
  • FREE positive control: HEK293T cell transient overexpression lysate (LC427274) , 20ug
100ul 325
$292
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WB(1)
IHC(2)
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Also for DNMT1 (NM_001130823)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Dnmt1 was used as an immunogen.
Clone NameEPR3521(2) IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; FC: 1:10 - 1:100
BufferDoes not react with Mouse, Rat. Is unsuitable for ICC/IF or IP.
Purification pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Note Is unsuitable for ICC/IF or IP.

Reference Data

Target NameHomo sapiens DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1
Alternative NameADCADN; AIM; CXXC9; DNMT; HSN1E; MCMT
Database LinkNP_001124295
FunctionDnmt1 is one of three families of enzymes (Dnmt1, Dnmt2, Dnmt3 (a and b)) that have a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Abnormal methylation patterns are associated with certain human tumors and developmental abnormalities (1). Dnmt1 is responsible for maintenance of the DNA methylation pattern after DNA replication. Dnmt1 is a large protein and can be subdivided into an N- and a C-terminal part. The C-terminal domain harbors the catalytic center. The N-terminal part of the enzyme comprises several individual domains, and plays a role in intracellular targeting and regulation of the enzyme's activity. Dnmt1 shows a preference for hemimethylated CG sites and the specificity of Dnmt1 for hemimethylated target sites is a key property for that function (2).
Related Pathway

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WB Image
Western blot - Anti-Dnmt1 antibody [EPR3521(2)]; All lanes : Anti-Dnmt1 antibody [EPR3521(2)] at 1/1000 dilution.Lane 1 : Jurkat cell lysates.Lane 2 : 293T cell lysates.Lane 3 : HeLa cell lysates.Lane 4 : HuT-78 cell lysates.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 183 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR3521(2)]; Immunohistochemical analysis of paraffin embedded Human stomach tissue labelled with TA307586 at 1/250 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR3521(2)]; Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelled with TA307586 at 1/250 dilution.

 

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