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Home Antibody All anti-DNAJA2 antibodies

Anti-DNAJA2 TRUEMAB Antibody clone 3H10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501739
  • DNAJA2 mouse monoclonal antibody, clone 3H10
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401779) , 20ug Explanation
100ul 379 In Stock
CF501739
  • Carrier-free (BSA/glycerol-free) DNAJA2 mouse monoclonal antibody, clone 3H10
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IHC(7)
IF(1)
FC(3)
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Also for DNAJA2 (NM_005880)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human DNAJA2 (NP_005871) produced in HEK293T cell.
Clone Nameclone 3H10 IsotypeIgG1
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.7 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:50~150, IF 1:100, FLOW 1:100
Predicted MW Explanation 45.6 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens DnaJ (Hsp40) homolog, subfamily A, member 2 (DNAJA2)
Alternative NameCPR3; DJ3; DJA2; DNAJ; DNJ3; HIRIP4; PRO3015; RDJ2
Database LinkNP_005871
Entrez Gene 10294 Human
Entrez Gene 84026 Rat
Entrez Gene 716353 Monkey
Entrez Gene 478143 Dog
FunctionThe protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJA2 (RC202204, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJA2.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJA2 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DNAJA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501739)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DNAJA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501739)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DNAJA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501739)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DNAJA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501739)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DNAJA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501739)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DNAJA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501739)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-DNAJA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501739)
IF Image
Anti-DNAJA2 mouse monoclonal antibody (TA501739) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJA2(RC202204).
FC Image
HEK293T cells transfected with either RC202204 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DNAJA2 antibody(TA501739), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Hela cells, using anti-DNAJA2 antibody(TA501739),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-DNAJA2 antibody(TA501739),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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