Anti-DNAJA2 Antibody, clone 3H10

Also for DNAJA2 (NM_005880)

Gene Name	Homo sapiens DnaJ (Hsp40) homolog, subfamily A, member 2 (DNAJA2)
Synonyms:	CPR3; DJ3; DJA2; DNAJ; DNJ3; HIRIP4; PRO3015; RDJ2
Immunogen	Full length human recombinant protein of human DNAJA2 (NP_005871) produced in HEK293T cell.
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 3H10	Isotype	IgG1
Species Reactivity	Human, Dog, Rat, Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:500~2000, IHC 1:50~150, IF 1:100, FLOW 1:100
Background	The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro. [provided by RefSeq].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJA2 (RC202204, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJA2.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJA2 monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DNAJA2 mouse monoclonal antibody. (TA501739)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DNAJA2 mouse monoclonal antibody. (TA501739)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DNAJA2 mouse monoclonal antibody. (TA501739)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DNAJA2 mouse monoclonal antibody. (TA501739)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DNAJA2 mouse monoclonal antibody. (TA501739)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DNAJA2 mouse monoclonal antibody. (TA501739)

IHC Image

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-DNAJA2 mouse monoclonal antibody. (TA501739)

IF Image

Anti-DNAJA2 mouse monoclonal antibody (TA501739) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJA2(RC202204).

FC Image

HEK293T cells transfected with either RC202204 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DNAJA2 antibody(TA501739), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Hela cells, using anti-DNAJA2 antibody(TA501739),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-DNAJA2 antibody(TA501739),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

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Anti-DNAJA2 TrueMAB Antibody clone 3H10

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