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Anti-DLG4 Antibody EP2652Y
Also for DLG4 (NM_001365)
|A synthetic peptide corresponding to residues in the N-terminus of human PSD95 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||ICC/IF: Use at an assay dependent concentration; WB: 1:1000 - 1:5000; IP: 1:10; ICC: 1:100 - 1:250; FC: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for IHC-P.
|Homo sapiens discs, large homolog 4 (Drosophila) (DLG4), transcript variant 1|
|PSD95; SAP-90; SAP90|
|This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
Wnt Signaling Pathway
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Western blot - PSD95 antibody [EP2652Y]; All lanes : Anti-PSD95 antibody [EP2652Y] at 1/2000 dilution.Lane 1 : Human brain tissue lysate.Lane 2 : Mouse brain tissue lysate.Lane 3 : Rat brain tissue lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 95 kDa.Observed band size : 95 kDa.
Flow Cytometry-Anti-PSD95 antibody [EP2652Y](TA303851); Overlay histogram showing SH-SY5Y cells stained with TA303851 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1Âµg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.