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Anti-DIAPH1 Antibody EPR7948
Also for DIAPH1 (NM_001079812)
|A synthetic peptide corresponding to residues in human Diap1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC/IF: 1:100 - 1:250
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens diaphanous-related formin 1 (DIAPH1), transcript variant 2|
|DFNA1; DIA1; DRF1; hDIA1; LFHL1|
|Diap1 is a homolog of Drosophila diaphanous , and it has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. Diap1 has therefore been speculated to have a role in the regulation of actin polymerization in hair cells of the inner ear (1).|
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Western blot - Anti-DIAPH1 antibody [EPR7948]; All lanes : Anti-DIAPH1 antibody [EPR7948] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : Human fetal brain lysate.Lane 4 : K562 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution.Predicted band size : 141 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH1 antibody [EPR7948]; ab129167, at 1/50 dilution staining DIAPH1 in paraffin-embedded Human stomach tissue, by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-DIAPH1 antibody [EPR7948]; ab129167, at 1/100 dilution, staining DIAPH1 in HeLa cells by Immunofluorescence.