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Anti-DIABLO Antibody Y12
Also for DIABLO (NM_138929)
|A synthetic peptide corresponding to C-terminal residues human Smac/DIABLO was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC: 1:10; IP: 1:10 - 1:80; FC: 1:10 - 1:50; ICC/IF: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Homo sapiens diablo, IAP-binding mitochondrial protein (DIABLO), nuclear gene encoding mitochondrial protein, transcript variant 3|
|DFNA64; DIABLO-S; SMAC; SMAC3|
|This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein. [provided by RefSeq]. |
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Western blot - Smac / Diablo antibody [Y12]; Anti-Smac / Diablo antibody [Y12] at 1/1000 dilution + Jurkat cell lysate.Predicted band size : 27 kDa.Observed band size : 21 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Smac / Diablo antibody [Y12]; ab32023, at a 1/10 dilution, staining human Smac/Diablo in skin cancer tissue by Immunohistochemistry, Paraffin embedded tissue
Immunocytochemistry/ Immunofluorescence - Anti-Smac / Diablo antibody [Y12]; Immunofluorescence analysis of IMR32 cells, staining Smac / Diablo with ab32023. Cells were fixed with 4% paraformaldehyde before incubating with primary antibody. An AlexaFluor?-conjugated anti-rabbit IgG was used as the secondary antibody.