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Home Antibody All anti-DHFR antibodies

Anti-DHFR Antibody EPR5285


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal antibody against DHFR
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400271) , 20ug Explanation
100ul $325 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart


OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human DHFR was used as an immunogen.
Clone NameEPR5285 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:10000 - 1:50000; IHC-P: 1:100 - 1:250; ICC: 1:25 - 1:50
Predicted MW Explanation 21 kDa
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens dihydrofolate reductase (DHFR), transcript variant 1
Alternative NameDHFRP1; DYR
Database LinkNP_000782
Entrez Gene 1719 Human
Entrez Gene 13361 Mouse
Entrez Gene 24312 Rat
FunctionDihydrofolate reductase (DHFR) converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. DHFR deficiency has been linked to megaloblastic anemia (1).
Related PathwayStem cell - PluripotencyDruggable Genome One carbon pool by folateFolate biosynthesisMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]; All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] at 1/10000 dilution.Lane 1 : HeLa cell lysates.Lane 2 : Jurkat cell lysates.Lane 3 : Y-79 cell lysates.Lane 4 : 293T cell lysates.Lane 5 : Mouse spleen lysates.Lane 6 : Rat kidney lysates.Lysates/proteins at 10 ug per lane.Predicted band size : 21 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]; TA310408 at 1/100 dilution, staining Dihydrofolate reductase (DHFR) in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.


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