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Anti-DHFR Antibody EPR5285
Also for DHFR (NM_000791)
|A synthetic peptide corresponding to residues in human DHFR was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:10000 - 1:50000; IHC-P: 1:100 - 1:250; ICC: 1:25 - 1:50
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens dihydrofolate reductase (DHFR), transcript variant 1|
|Dihydrofolate reductase (DHFR) converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. DHFR deficiency has been linked to megaloblastic anemia (1).|
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Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]; All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] at 1/10000 dilution.Lane 1 : HeLa cell lysates.Lane 2 : Jurkat cell lysates.Lane 3 : Y-79 cell lysates.Lane 4 : 293T cell lysates.Lane 5 : Mouse spleen lysates.Lane 6 : Rat kidney lysates.Lysates/proteins at 10 µg per lane.Predicted band size : 21 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]; TA310408 at 1/100 dilution, staining Dihydrofolate reductase (DHFR) in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.