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Anti-DFNA5 GAT Antibody Polyclonal
Generated by Genomic Immunization
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Also for DFNA5 (NM_004403)
|DNA immunization. This antibody is specific for the C Terminus Region of the target protein.|
|Human , Dog , Mouse
|WB, ELISA, IHC, IF
||WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens deafness, autosomal dominant 5 (DFNA5), transcript variant 1|
Entrez Gene 1687 Human
Entrez Gene 54722 Mouse
Entrez Gene 611223 Dog
|Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]|
|Druggable Genome |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFNA5 (RC210515, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFNA5.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DFNA5 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA: Dfna5 deafness Antibody - Affinity Purified
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-DFNA5 rabbit polyclonal antibody. (TA590634)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-DFNA5 rabbit polyclonal antibody. (TA590634)
Immunohistochemistry: Dfna5 deafness Antibody - Most of the normal cells showed strong cytoplasmic positivity. Glial cells displayed moderate cytoplasmic staining in a fraction of cells. A majority of the malignant cells displayed moderate cytoplasmic immunoreactivity. Several cases of lymphomas, melanomas, thyroid, colorectal, ovarian, endometrial cancers and cholangiocarcinomas showed strong positivity. Image and statement courtesy of the Human Protein Atlas (HPA).
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.