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Anti-DFFA Antibody EPR4220
Also for DFFA (NM_004401)
|A synthetic peptide corresponding to residues in human DFF45/DFF35 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:500 - 1:1000; ICC: 1:250 - 1:500; FC: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1|
|DFF-45; DFF1; ICAD|
|DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kDa (DFF40) and 45-kDa (DFF45) subunits. DFF45 is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFF45 is cleaved by caspase-3. The cleaved fragments of DFF45 dissociate from DFF40, the active component of DFF. DFF40 has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.|
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Western blot - ICAD antibody [EPR4220]; All lanes : Anti-ICAD antibody [EPR4220] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HeLa cell lysate.Lysates/proteins at 10 µg/ml per lane.Predicted band size : 37 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ICAD antibody [EPR4220]; Immunohistochemical staining of paraffin-embedded human breast carcinoma using ab108924 at a diltion of 1/500.
Immunocytochemistry/ Immunofluorescence - ICAD antibody [EPR4220]; Immunofluorescent staining of HeLa cells using ab108924 at a dilution of 1/250.