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Home Antibody All anti-DES antibodies

Anti-DES Antibody Y66

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300414
  • Rabbit Monoclonal Antibody against DES (Clone Y66)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400713) , 20ug
100ul 325 3-7 Days
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WB(2)
IHC(8)
IF(2)
FC(1)
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Also for DES (NM_001927)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to C-terminus of human Desmin was used as immunogen. Predicted to cross-react with rat, bovine and pig, based on sequence homology.
Clone NameY66 IsotypeIgG
Species ReactivityMouse, Rat, Guinea pig, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: Use at an assay dependent concentration; WB: 1:500; IHC-P: Use at an assay dependent dilution; IHC - Wholemount: Use at an assay dependent concentration; FC: 1:10 - 1:100; IHC-Fr: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens desmin (DES)
Alternative NameCSM1; CSM2; LGMD2R
Database LinkNP_001918
FunctionDesmin are class-III intermediate filaments found in muscle cells, and are expressed during the formation of muscle cells or fibers. Cytoskeletal intermediate filaments (IFs) are constructed from two-chain alpha-helical coiled-coil molecules arranged on an imperfect helical lattice (1,2). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. Desmin mutations have been linked to familial cardiac and skeletal myopathy (3,4).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Desmin antibody [Y66]; All lanes : Anti-Desmin antibody [Y66] at 1/500 dilution.Lane 1 : Human skeletal muscle whole tissue lysate.Lane 2 : Human skeletal muscle whole tissue lysate.Lane 3 : Human skeletal muscle whole tissue lysate.Lane 4 : Human skeletal muscle whole tissue lysate.Lysates/proteins at 20 ug per lane.Secondary.IRDye 680-conjugated anti-rabbit at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 53 kDa.Observed band size : 53 kDa.Exposure time : 50 seconds
WB Image
Western blot - Anti-Desmin antibody [Y66]; Anti-Desmin antibody [Y66] at 1/500 dilution + mouse heart tissue.Predicted band size : 53 kDa.Observed band size : 55,60 kDa .
IHC Image
Anti-Desmin antibody [Y66]; Immunohistochemical analysis of Desmin expression in human heart muscle using 1/500 TA300414.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66]; TA300414 staining Desmin in nude rat esophagheal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6. Samples were then blocked with 1% BSA for 20 minutes at 25°C and then incubated with TA300414 at a 1/400 dilution for 16 hours at 25°C. The secondary used was an undiluted goat anti-rabbit HRP conjugated polyclonal..Striated muscle cells of muscular propria are strongly positive for desmin. Smooth muscle cells and vascular smooth muscle cells in submucosal layer are also positive for desmin.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66]; TA300414 showing positive staining in Normal uterus tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66]; TA300414 showing positive staining in Normal urinary bladder tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66]; TA300414 showing positive staining in skeletal muscle tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66]; TA300414 showing negative staining in Normal brain tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66]; TA300414 showing negative staining in Normal tonsil tissue.
IHC Image
Immunohistochemistry (Frozen sections) - Anti-Desmin antibody [Y66]; Immunohistochemical analysis of mouse skeletal muscle tissue following cardiotoxin injury, staining Desmin with TA300414.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Desmin antibody [Y66]; TA300414 staining Desmin (green) inHuman skeletal muscle cellsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methacarn and blocked with 10% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/150) for 12 hours. An Alexa Fluor? 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Blue - DAPI-nuclei. Red - WGA. 40x objective.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Desmin antibody [Y66]; TA300414 stainingDesmin (green) inMouse aorta smooth muscle cellsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formalin and blocked with 10%serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/150) for 1 hour at 22°C. An Alexa Fluor? 488-conjugated Goat anti-rabbit IgG polyclonal (1/200)was used as the secondary antibody. Blue - nuclei.
FC Image
Flow Cytometry - Anti-Desmin antibody [Y66]; Flow cytometric analysis of permeabilized Hela cells using anti-Desmin TA300414 (red line) or a rabbit IgG (negative) (green line). used in 1:20 dilution. FITC conjugated Goat anti Rabbit IgG (H+L) used as secondary.

 

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