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Anti-DEF6 Antibody EPR7492
Also for DEF6 (NM_022047)
|A synthetic peptide corresponding to residues on the C-terminus in human DEF6 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, FC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; ICC: 1:100 - 1:250; FC: 1:100 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for IHC-P.|
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for IHC-P.
|Homo sapiens differentially expressed in FDCP 6 homolog (mouse) (DEF6)|
|IBP; SLAT; SWAP70L|
|DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC and CDC42 that is highly expressed in B and T cells (1). It plays a unique role in TCR-mediated signaling pathways and the control of adaptive immune responses (2). DEF6 is also involved in the development and maturation of Th2 cells (3).|
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Western blot - Anti-DEF6 antibody [EPR7492]; All lanes : Anti-DEF6 antibody [EPR7492] at 1/1000 dilution.Lane 1 : Human tonsil lysate.Lane 2 : Ramos lysate.Lane 3 : MOLT4 lysate.Lane 4 : Human thymus lysate.Lane 5 : Jurkat lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 74 kDa.
Other-Anti-DEF6 antibody [EPR7492](ab126792); Equilibrium disassociation constant (KD)..
Flow Cytometry - Anti-DEF6 antibody [EPR7492]; ab126792 at 1/100 dilution staining DEF6 in permeabilized Jurkat cells by Flow cytometry (red) compared with a rabbit IgG negative control (green).