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Anti-DDT TRUEMAB Antibody Clone OTI3A11
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Full length human recombinant protein of human DDT(NP_001346) produced in HEK293T cell.|
|WB, IHC, IF
|| WB 1:8000, IHC 1:150, IF 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens D-dopachrome tautomerase (DDT), transcript variant 1|
Entrez Gene 1652 Human
|D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq, Jul 2008]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDT (RC202047, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDT.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DDT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DDT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505602)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DDT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505602)
Immunofluorescent staining of HeLa cells using anti-DDT mouse monoclonal antibody (TA505602).