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Anti-DCTN3 Antibody EPR5097


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal antibody against DCTN3
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416118) , 20ug Explanation
100ul 325 3-7 Days
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Also for DCTN3 (NM_007234)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human DCTN3 was used as an immunogen.
Clone NameEPR5097 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250
Predicted MW Explanation 21 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC or IP.

Reference Data

Target NameHomo sapiens dynactin 3 (p22) (DCTN3), transcript variant 1
Alternative NameDCTN-22; DCTN22
Database LinkNP_009165
Entrez Gene 11258 Human
Entrez Gene 53598 Mouse
Entrez Gene 362504 Rat
FunctionDCTN3 is the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kDa. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. DCTN3 is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin (1).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-DCTN3 antibody [EPR5097]; All lanes : Anti-DCTN3 antibody [EPR5097] at 1/1000 dilution.Lane 1 : 293T cell lysates.Lane 2 : Mouse muscle lysates.Lane 3 : HeLa cell lysates.Lane 4 : BxPC 3 cell lysates.Lane 5 : SH-SY5Y cell lysates.Lane 6 : Rat muscle lysates.Lysates/proteins at 10 ug per lane.Secondary.Lanes 1, 2, 3, 5 & 6 : Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 21 kDa.
Assay Image
Other-Anti-DCTN3 antibody [EPR5097](TA310399); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097]; TA310399, at 1/100 dilution, staining DCTN3 in paraffin embedded Human breast tissue by Immunohistochemistry.


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