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Anti-DCLK3 GAT Antibody Polyclonal
Generated by Genomic Immunization
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Also for DCLK3 (NM_033403)
|DNA immunization. This antibody is specific for the N Terminus Region of the target protein.|
|WB, ELISA, IHC
||WB: 1:5000-1:20000; ELISA: 1:100-1:2000; IHC: 1:10-1:2000; IHC-P 1:10-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
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Western Blot: DCLK3 Antibody - Western Blot was performed using affinity purified SEQer DCAMKL3 antibody, aa(138-237) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer DCAMKL3 antibody, aa(138-237) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
Western Blot: DCLK3 Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight: 74 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.
Immunohistochemical staining of human cerebellum shows cytoplasmic and nuclear positivity in Purkinje cells. Antibody dilution: 1:400. Image and statement courtesy of the Human Protein Atlas (HPA).