Home Antibody All anti-DCLK3 antibodies
Anti-DCLK3 GAT Antibody Polyclonal
UltraMAB Antibodies - Validated with 10K Protein Chip
Also for DCLK3 (NM_033403)
|DNA immunization. This antibody is specific for the N Terminus Region of the target protein.|
|WB, ELISA, IHC
||WB: 1:5000-1:20000; ELISA: 1:100-1:2000; IHC: 1:10-1:2000; IHC-P 1:10-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody. (Protein A or G Sepharose)
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens doublecortin-like kinase 3 (DCLK3)|
|CLR; DCAMKL3; DCDC3C; DCK3|
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Western Blot: DCLK3 Antibody - Western Blot was performed using affinity purified SEQer DCAMKL3 antibody, aa(138-237) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer DCAMKL3 antibody, aa(138-237) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
Western Blot: DCLK3 Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight: 74 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.
Immunohistochemistry: DCLK3 Antibody - Normal tissues generally displayed weak to moderate cytoplasmic and occasional nuclear positivity. Ciliated cells in airways and fallopian tube exhibited distinct membranous staining. Cells for example in liver, prostate and spleen were negative. Colo-rectal and endometrial cancers exhibited some moderately stained cases while malignant gliomas, ovarian and stomach cancers showed several weakly immunoreactive samples. Remaining malignant tissues were mainly negative. Image and statement courtesy of the Human Protein Atlas (HPA).