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Anti-DCK TRUEMAB Antibody Clone OTI3F5
TrueMAB Antibodies - Made against Authentic Protein Antigens
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|Full length human recombinant protein of human DCK (NP_000779) produced in HEK293T cell. |
|WB, IHC, IF, FC
|| WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens deoxycytidine kinase (DCK)|
Entrez Gene 1633 Human
|Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq, Jul 2008]. |
|Druggable Genome Purine metabolismPyrimidine metabolism|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCK (RC210767, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCK.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DCK monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502688)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502688)
Anti-DCK mouse monoclonal antibody (TA502688) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCK(RC210767).
HEK293T cells transfected with either RC210767 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DCK antibody(TA502688), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-DCK antibody(TA502688),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-DCK antibody(TA502688),(Red), compared to a nonspecific negative control antibody,(Blue).