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Anti-DCK TRUEMAB Antibody clone 3F5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502688
  • DCK mouse monoclonal antibody, clone 3F5
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400272) , 20ug Explanation
100ul $325 In Stock
CF502688
  • Carrier-free (BSA/glycerol-free) DCK mouse monoclonal antibody, clone 3F5
100ug $450 3-4 weeks
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WB(1)
IHC(2)
IF(1)
FC(3)

OriGene Data

ImmunogenFull length human recombinant protein of human DCK (NP_000779) produced in HEK293T cell.
Clone Nameclone 3F5 IsotypeIgG2a
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested Dilutions WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 30.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens deoxycytidine kinase (DCK)
Alternative NameMGC117410; MGC138632
Database LinkNP_000779
Entrez Gene 1633 Human
FunctionDeoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq, Jul 2008].
Related PathwayDruggable Genome Purine metabolismPyrimidine metabolism

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCK (RC210767, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCK.
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-DCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502688)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502688)
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Anti-DCK mouse monoclonal antibody (TA502688) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCK(RC210767).
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HEK293T cells transfected with either RC210767 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DCK antibody(TA502688), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-DCK antibody(TA502688),(Red), compared to a nonspecific negative control antibody,(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-DCK antibody(TA502688),(Red), compared to a nonspecific negative control antibody,(Blue).
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