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Home Antibody All anti-DBT antibodies

Anti-DBT TRUEMAB Antibody Clone 1G2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA504748
  • DBT mouse monoclonal antibody,clone 1G2
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419653) , 20ug Explanation
100ul 379 In Stock
CF504748
  • Carrier-free (BSA/glycerol-free) DBT mouse monoclonal antibody,clone 1G2
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(1)
IF(1)
FC(1)
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Also for DBT (NM_001918)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human DBT(NP_001909) produced in HEK293T cell.
Clone NameClone 1G2 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:2000, FLOW 1:100,
Predicted MW Explanation 46.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens dihydrolipoamide branched chain transacylase E2 (DBT)
Alternative NameBCATE2; BCKAD-E2; BCKADE2; E2; E2B
Database LinkNP_001909
Entrez Gene 1629 Human
FunctionThe branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq].
Related PathwayDruggable Genome Valine, leucine and isoleucine degradationMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBT (RC201998, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBT.
IF Image
Anti-DBT mouse monoclonal antibody (TA504748) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DBT(RC201998).
FC Image
HEK293T cells transfected with either RC201998 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DBT antibody(TA504748), and then analyzed by flow cytometry.

 

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