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Anti-DAXX Antibody E94
Also for DAXX (NM_001350)
|A synthetic peptide corresponding to residues of human DAXX was used as immunogen. Predicted to cross-react with rat, based on sequence homology.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:5000; IHC-P: Use at an assay dependent concentration; ICC: 1:50; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for IP.
|Homo sapiens death-domain associated protein (DAXX), transcript variant 2|
|BING2; DAP6; EAP1|
Entrez Gene 1616 Human
|Daxx is a nuclear protein that has been found to the enhance Fas-mediated apoptosis and activate the JNK pathway (1). Apoptosis signal-regulating kinase 1 (ASK1) controls the dual function of Daxx as a transcriptional repressor in the nucleus and as a proapoptotic signal mediator in the cytoplasm (2). Daxx associates with various proteins, including promyelocytic leukemia protein (PML) and heat shock protein 27, lending significance to their roles in tumor suppression and Daxx-mediated apoptosis (3,4).|
|Stem cell - PluripotencyTranscription FactorsDruggable Genome MAPK signaling pathwayAmyotrophic lateral sclerosis (ALS)|
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Western blot - Daxx antibody [E94]; Anti-Daxx antibody [E94] at 1/5000 dilution + Hela cell lysate.Predicted band size : 81 kDa.Observed band size : 100 kDa .Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - Daxx antibody [E94]; Ab32140, at a dilution of 1/50, staining Daxx in paraffin embedded human stomach adenocarcinoma tissue by Immunohistochemistry.
Flow Cytometry - Anti-Daxx antibody; Overlay histogram showing HeLa cells stained with TA300370 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.