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Home Antibody All anti-DAXX antibodies

Anti-DAXX Antibody E94

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300370
  • Rabbit Monoclonal Antibody against DAXX (Clone E94)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400540) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for DAXX (NM_001350)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues of human DAXX was used as immunogen. Predicted to cross-react with rat, based on sequence homology.
Clone NameE94 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:5000; IHC-P: Use at an assay dependent concentration; ICC: 1:50; FC: 1:100
BufferDoes not react with Mouse, Rat. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens death-domain associated protein (DAXX), transcript variant 2
Alternative NameBING2; DAP6; EAP1
Database LinkNP_001341
Entrez Gene 1616 Human
FunctionDaxx is a nuclear protein that has been found to the enhance Fas-mediated apoptosis and activate the JNK pathway (1). Apoptosis signal-regulating kinase 1 (ASK1) controls the dual function of Daxx as a transcriptional repressor in the nucleus and as a proapoptotic signal mediator in the cytoplasm (2). Daxx associates with various proteins, including promyelocytic leukemia protein (PML) and heat shock protein 27, lending significance to their roles in tumor suppression and Daxx-mediated apoptosis (3,4).
Related PathwayStem cell - PluripotencyTranscription FactorsDruggable Genome MAPK signaling pathwayAmyotrophic lateral sclerosis (ALS)

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Daxx antibody [E94]; Anti-Daxx antibody [E94] at 1/5000 dilution + Hela cell lysate.Predicted band size : 81 kDa.Observed band size : 100 kDa .Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Daxx antibody [E94]; Ab32140, at a dilution of 1/50, staining Daxx in paraffin embedded human stomach adenocarcinoma tissue by Immunohistochemistry.
FC Image
Flow Cytometry - Anti-Daxx antibody [E94]; Overlay histogram showing HeLa cells stained with TA300370 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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