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Home Antibody All anti-DAPK2 antibodies

Anti-DAPK2 TRUEMAB Antibody clone 1B5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501088
  • DAPK2 (DAP Kinase 2 ) mouse monoclonal antibody, clone 1B5
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402315) , 20ug Explanation
100ul 325 In Stock
CF501088
  • Carrier-free (BSA/glycerol-free) DAPK2 mouse monoclonal antibody, clone 1B5
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(1)
IP(1)
IHC(3)
IF(1)
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Also for DAPK2 (NM_014326)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human DAPK2 (NP_055141) produced in HEK293T cell.
Clone Nameclone 1B5 IsotypeIgG2b
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF, IP Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, IP 2ug/500ul
Predicted MW Explanation 42.7 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens death-associated protein kinase 2 (DAPK2)
Alternative NameDRP-1; DRP1
Database LinkNP_055141
Entrez Gene 23604 Human
FunctionThis gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq].
Related PathwayProtein KinaseDruggable Genome Pathways in cancerBladder cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAPK2 (RC216274, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAPK2.
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501088)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501088)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501088)
IF Image
Anti-DAPK2 mouse monoclonal antibody (TA501088) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DAPK2(RC216274).
IP Image
Immunoprecipitation(IP) of DAPK2 by using TrueMab monoclonal anti-DAPK2 antibodies (Negative control: IP without adding anti-DAPK2 antibody.). For each experiment, 500ul of DDK tagged DAPK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-DAPK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

 

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