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Home Antibody All anti-DAPK2 antibodies

Anti-DAPK2 Antibody EP1633Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300907
  • Rabbit Monoclonal Antibody against DAPK2 (Clone EP1633Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402315) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for DAPK2 (NM_014326)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the N-terminus of human DAPK2 was used as an immunogen.
Clone NameEP1633Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: Use at an assay dependent concentration; FC: 1:1000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC/IF or IP.

Reference Data

Target NameHomo sapiens death-associated protein kinase 2 (DAPK2)
Alternative NameDRP-1; DRP1
Database LinkNP_055141
FunctionProtein kinase termed death-associated protein kinase 2 (DAPK2) dependant on calcium/calmodulin (Ca2+/CaM) contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a protein kinase involved in apoptosis. Overexpression of DAPK2 significantly induced the morphological changes characteristic of apoptosis. Results indicate that DAPK2 is an additional member of DAP kinase family involved in apoptotic signaling (1). The region of homology spans the catalytic domain and the CaM-regulatory region, whereas the remaining C-terminal part of the protein differs completely from DAP kinase and displays no homology to any known protein. The catalytic domain is also homologous to the recently identified ZIP kinase and to a lesser extent to the catalytic domains of DRAK1 and -2 (2). DAPK2 has a novel regulatory mechanism that controls its pro-apoptotic functions. It comprises a single autophosphorylation event mapped to Ser308 within the CaM regulatory domain. A negative charge at this site reduces both the binding to CaM and the formation of DRP-1 homodimers (3).
Related Pathway

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WB Image
Western blot - DAP Kinase 2 antibody [EP1633Y]; Anti-DAP Kinase 2 antibody [EP1633Y] at 1/2000 dilution + Rat spleen lysate at 10 µg.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 42 kDa.Observed band size : 42 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - DAP Kinase 2 antibody [EP1633Y]; Immunohistochemical staining of paraffin embedded human liver carcinoma using TA300907 (1/50). Secondary stained using Goat anti-rabbit HRP (1/2000).
FC Image
Flow Cytometry - Anti-DAP Kinase 2 antibody [EP1633Y]; Overlay histogram showing HeLa cells stained with TA300907 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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