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Home Antibody All anti-DAB2 antibodies

Anti-DAB2 Antibody EP2297Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301090
  • Rabbit Monoclonal Antibody against DAB2 (Clone EP2297Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400534) , 20ug
100ul 325 In Stock
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WB(1)
FC(1)
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Also for DAB2 (NM_001343)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human Dab2 was used as an immunogen.
Clone NameEP2297Y IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:2000 - 1:10000; IP: 1:20; ICC: 1:100 - 1:250; FC: 1:50
BufferDoes not react with Mouse, Rat. Is unsuitable for IHC-P.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IHC-P.

Reference Data

Target NameHomo sapiens Dab, mitogen-responsive phosphoprotein, homolog 2 (Drosophila) (DAB2), transcript variant 1
Alternative NameDOC-2; DOC2
Database LinkNP_001334
FunctionDab2 (Disabled homolog 2, Diabled-2) is a mammalian structural homolog of Drosophila Disabled protein and a mitogen-responsive phosphoprotein (1). As a component of the CSF1 signal transduction pathway, Dab2 is normally expressed in ovarian epithelia cells. However, in ovarian carcinoma, Dab2 expression is either lost or down-regulated. Therefore, Dab2 is speculated to be a negative regulator of growth and its down-regulation considered to play critical role in ovarian carcinogenesis (2).
Related Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - DAB2 antibody [EP2297Y]; Anti-DAB2 antibody [EP2297Y] at 1/2000 dilution + human pancreas tissue lysates at 10 ug.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 83 kDa.Observed band size : 105 kDa .
FC Image
Flow Cytometry-Anti-DAB2 antibody [EP2297Y](TA301090); Overlay histogram showing HeLa cells stained with TA301090 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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