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Anti-Cebpb Antibody E299


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against C/EBP ß (C-term)(clone E299)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417451) , 20ug Explanation
100ul $325 2 Weeks
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Also for Cebpb (NM_005194)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in C-terminus of rat C/EBP b was used as immunogen. The antibody can recognize three C/EBPb isoform (i.e. LAP*, LAP, and LIP).
Clone NameE299 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsEMSA: Use at an assay dependent concentration; WB: 1:1000; ICC/IF: 1:50 - 1:100; FC: 1:1000; IP: 1:20
Predicted MW Explanation 36 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IHC-P.

Reference Data

Target NameHomo sapiens CCAAT/enhancer binding protein (C/EBP), beta (CEBPB), transcript variant 1
Alternative NameC/EBP-beta; CRP2; IL6DBP; NF-IL6; TCF5
Database LinkNP_005185
Entrez Gene 1051 Human
Entrez Gene 12608 Mouse
Entrez Gene 24253 Rat
FunctionCCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth & differentiation. The various isoforms of C/EBP (a,ß,?,d,e, ? ) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). A number of serine targets of PKA have been identified in C/EBP-ß, suggesting its activities are post-translationally regulated by cAMP (2). Even though, it has been show that C/EBP ß occurs predominantly as a heterodimer (3), C/EBP ß & ? readily heterodimerize with each other as well as with C/EBP a (4).
Related PathwayES Cell Differentiation/IPSTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - CEBPB antibody [E299]; Anti-CEBP Beta antibody [E299] at 1/1000 dilution + PC12 cell lysate.Predicted band size : 36 kDa.
Western blot - CEBP Beta antibody [E299]; All lanes : Anti-CEBP Beta antibody [E299] at 1/500 dilution.Lane 1 : 3T3 Cells.Lane 2 : MCF7 Cells.Lane 3 : PC12 Cells.Lane 4 : Rat Spleen Lysate.Lane 5 : Rat Kidney Lysate.Lane 6 : Rat Heart Lysate.Lane 7 : Rat Brain Lysate.Lane 8 : Mouse Spleen Lysate.Lane 9 : Mouse Kidney Lysate.Lane 10 : Mouse Heart Lysate.Lane 11 : Mouse Brain Lysate.Predicted band size : 36 kDa.
Immunocytochemistry/ Immunofluorescence - CEBPB antibody [E299]; Ab32358, at a 1/50 dilution, staining CEBPB in Hela cells by Immunofluorescence.
Flow Cytometry - Anti-CEBP Beta antibody [E299]; Overlay histogram showing HeLa cells stained with TA307242 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


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