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Home Antibody All anti-CYP27A1 antibodies

Anti-CYP27A1 Antibody EPR7529

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310387
  • Rabbit Monoclonal antibody against CYP27A1
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400268) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(2)
IF(1)
Assay(1)
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Also for CYP27A1 (NM_000784)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human CYP27A1 was used as an immunogen.
Clone NameEPR7529 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:50 - 1:100
Predicted MW Explanation 60 kDa
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens cytochrome P450, family 27, subfamily A, polypeptide 1 (CYP27A1)
Alternative NameCP27; CTX; CYP27
Database LinkNP_000775
Entrez Gene 1593 Human
Entrez Gene 104086 Mouse
Entrez Gene 301517 Rat
FunctionCYP27A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, CYP27A1 is important for overall cholesterol homeostasis. Mutations in CYP27A1 cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease (1).
Related PathwayP450Druggable Genome Primary bile acid biosynthesisMetabolic pathwaysPPAR signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-CYP27A1 antibody [EPR7529]; All lanes : Anti-CYP27A1 antibody [EPR7529] at 1/1000 dilution.Lane 1 : HepG2 lysate.Lane 2 : Caco 2 lysate.Lane 3 : Human fetal liver lysate.Lane 4 : Mouse liver lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 60 kDa.
Assay Image
Other-Anti-CYP27A1 antibody [EPR7529](TA310387); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529]; TA310387, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529]; TA310387, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human liver tissue by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-CYP27A1 antibody [EPR7529]; TA310387, at a 1/50 dilution, staining CYP27A1 in HepG2 cells by Immunofluorescence.

 

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