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Anti-CYP27A1 Antibody EPR7529
Also for CYP27A1 (NM_000784)
|A synthetic peptide corresponding to residues in human CYP27A1 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:50 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens cytochrome P450, family 27, subfamily A, polypeptide 1 (CYP27A1)|
|CP27; CTX; CYP27|
Entrez Gene 1593 Human
Entrez Gene 104086 Mouse
Entrez Gene 301517 Rat
|CYP27A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, CYP27A1 is important for overall cholesterol homeostasis. Mutations in CYP27A1 cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease (1).|
|P450Druggable Genome Primary bile acid biosynthesisMetabolic pathwaysPPAR signaling pathway|
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Western blot - Anti-CYP27A1 antibody [EPR7529]; All lanes : Anti-CYP27A1 antibody [EPR7529] at 1/1000 dilution.Lane 1 : HepG2 lysate.Lane 2 : Caco 2 lysate.Lane 3 : Human fetal liver lysate.Lane 4 : Mouse liver lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 60 kDa.
Other-Anti-CYP27A1 antibody [EPR7529](TA310387); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529]; TA310387, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529]; TA310387, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human liver tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-CYP27A1 antibody [EPR7529]; TA310387, at a 1/50 dilution, staining CYP27A1 in HepG2 cells by Immunofluorescence.