Anti-CYP1A2 Antibody, clone 8F1

Also for CYP1A2 (NM_000761)

OriGene Data

Immunogen	Full length human recombinant protein of human CYP1A2 (NP_000752) produced in HEK293T cell.
Clone Name	clone 8F1	Isotype	IgG2a
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens cytochrome P450, family 1, subfamily A, polypeptide 2 (CYP1A2)
Alternative Name	CP12; P3-450; P450(PA)
Database Link	NP_000752
Function	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region.
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP1A2 (RC221636, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP1A2.

IHC Image

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-CYP1A2 mouse monoclonal antibody. (TA501196, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CYP1A2 mouse monoclonal antibody. (TA501196, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CYP1A2 mouse monoclonal antibody. (TA501196, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-CYP1A2 mouse monoclonal antibody. (TA501196, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CYP1A2 mouse monoclonal antibody. (TA501196, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-CYP1A2 mouse monoclonal antibody. (TA501196, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CYP1A2 mouse monoclonal antibody. (TA501196, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CYP1A2 mouse monoclonal antibody. (TA501196, Dilution 1:50)

IF Image

Anti-CYP1A2 mouse monoclonal antibody (TA501196) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP1A2(RC221636).

FC Image

HEK293T cells transfected with either RC221636 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CYP1A2 antibody(TA501196), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-CYP1A2 antibody(TA501196),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Anti-CYP1A2 TrueMAB Antibody clone 8F1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data