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Home Antibody All anti-CYB5R3 antibodies

Anti-CYB5R3 TRUEMAB Antibody Clone OTI2A10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA501353 CYB5R3 mouse monoclonal antibody, clone OTI2A10 (formerly 2A10) 100ul $379 In Stock
LC400140 CYB5R3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501353 Carrier-free (BSA/glycerol-free) CYB5R3 mouse monoclonal antibody, clone OTI2A10 (formerly 2A10) 100ug $450 3-4 weeks
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WB(2)
IHC(10)
FC(2)
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Also for CYB5R3 (NM_000398)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human CYB5R3 (NP_000389) produced in HEK293T cell.
Clone NameClone OTI2A10 IsotypeIgG2b
Species ReactivityHuman , Dog , Monkey Concentration0.76 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:200~500, IHC 1:150, FLOW 1:100
Predicted MW Explanation 34.1 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens cytochrome b5 reductase 3 (CYB5R3), transcript variant 1
Alternative NameB5R; DIA1
Database LinkNP_000389
Entrez Gene 1727 Human
Entrez Gene 714058 Monkey
Entrez Gene 474479 Dog
FunctionThis gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq].
Related PathwayDruggable Genome Amino sugar and nucleotide sugar metabolism

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R3 (RC201592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CYB5R3 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501353)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501353)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501353)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501353)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501353)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501353)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501353)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501353)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501353)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501353)
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HEK293T cells transfected with either RC201592 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CYB5R3 antibody(TA501353), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Jurkat cells, using anti-CYB5R3 antibody(TA501353),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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