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Anti-CXCR2 PHOSPHO Antibody
Also for CXCR2 (NM_001557)
|The antiserum was produced against synthesized phosphopeptide derived from human IL-8R beta/CDw128 beta around the phosphorylation site of serine 347 (R-P-SP-F-V).|
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:1000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody detects endogenous levels of IL-8R beta/CDw128 beta only when phosphorylated at serine347.
|Homo sapiens chemokine (C-X-C motif) receptor 2 (CXCR2), transcript variant 1|
|CD182; CDw128b; CMKAR2; IL8R2; IL8RA; IL8RB|
Cytokine-cytokine receptor interaction
Signaling by GPCR
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Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using IL-8R beta/CDw128 beta (Phospho-Ser347) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using IL-8R beta/CDw128 beta (Phospho-Ser347) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of COS7 cells, using IL-8R beta/CDw128 beta (Phospho-Ser347) antibody.The picture on the right is treated with the synthesized peptide.