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Anti-CUL3 Antibody EPR3196Y
Also for CUL3 (NM_003590)
|A synthetic peptide corresponding to residues on the C terminus of human CUL3 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:20000 - 1:100000; IP: 1:40; FC: 1:30 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for ICC or IHC-P.
|Homo sapiens cullin 3 (CUL3), transcript variant 1|
|Cullin proteins assemble a large number of RING E3 ubiquitin ligases and regulate various physiological processes (1). Cullin 3-based E3 ligase (CUL3) forms a catalytically inactive BTB-CUL3-Rbx1 (BCR) ubiquitin ligase, which becomes functional upon covalent attachment of the ubiquitin Nedd8 (neural precursor cell-expressed, developmentally downregulated 8) near the C terminus of CUL3 (2). CUL3 plays an essential role in both axonal arborization and proper elaboration of dendrites and may require neddylation for its proper function (3). The CUL3 pathway, which selects cyclin E for ubiquitination on the basis of its assembly into CDK complexes, may be one way to control cyclin abundance (4). In a complex with the substrate-specific adaptors KLHL9 and KLHL13, CUL3 is required for correct chromosome alignment in metaphase, proper midzone and midbody formation, and completion of cytokinesis (5). |
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Western blot - Cullin 3 antibody [EPR3196Y]; All lanes : Anti-Cullin 3 antibody [EPR3196Y] at 1/20000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : SH-SY5Y cell lysate.Lane 3 : PC12 cell lysate.Lane 4 : 3T3 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP-labeled goat anti-rabbit at 1/2000 dilution.Predicted band size : 89 kDa.Observed band size : 82,90 kDa .
Flow Cytometry - Anti-Cullin 3 antibody [EPR3196Y]; Overlay histogram showing HeLa cells stained with TA301317 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.