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Anti-CUL1 Antibody EPR3102Y
Also for CUL1 (NM_003592)
|A synthetic peptide corresponding to residues in human CUL1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1: 1000~2000; ICC: 1: 100~250; FC: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens cullin 1 (CUL1)|
|Cullin proteins assemble a large number of RING E3 ubiquitin ligases and regulate various physiological processes (1). It has been shown that the SKP1, CUL1, F-box (SCF) containing pathway, although similarly used by the mammalian CUL1, is not shared by other cullin members. This implies that most cullins may use a SKP1/F-box-independent pathway to facilitate protein degradation (2). The majority of CUL1 has been found in a complex with CAND1 and ROC1, independent of SKP1 and F-box protein SKP2. Data suggests that CAND1 regulates the formation of the SCF complex, and its dissociation from CUL1 is coupled with the incorporation of F-box proteins into the SCF complex, causing their destabilization (3). |
Delta-Notch Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot - Cullin 1 antibody [EPR3102Y]; All lanes : Anti-Cullin 1 antibody [EPR3102Y] at 1/2000 dilution.Lane 1 : 293T cell lysate.Lane 2 : A549 cell lysate.Lane 3 : T47D cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 90 kDa.Observed band size : 90 kDa.
Immunocytochemistry/ Immunofluorescence - Cullin 1 antibody [EPR3102Y]; ab75812 at 1/100 dilution staining Cullin 1 in HeLa cells by Immunofluorescence.