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b-Catenin UltraMAB Antibody, Clone UMAB14
UltraMAB Antibodies - Validated with 10K Protein Chip
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Also for b-Catenin (NM_001904)
|Full length human recombinant protein of human CTNNB1 (NP_001895) produced in HEK293T cell.|
|Human , Dog , Rat , Monkey , Mouse
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC, 10K-CHIP
||WB 1:500~2000, IHC 1:150, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 1|
|armadillo; CTNNB; MRD19|
|The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene. |
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Western analysis of HEK 293T cells without treatment or treated with 30mM LiCl for 16 hours, using anti-beta-catenin antibody (clone UMAB14). Anti-HSP90 (TA500494) was used as internal loading control.
Western Blot analysis of 10 different human tissue lysates (10ug) by using Anti-ß-Catenin monoclonal antibody (Clone UMAB14, 1:500)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody (Clone UMAB14).
Immunohistochemical staining of paraffin-embedded Mouse colon tissue using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB14, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded human normal ovary and Adenocarcinoma of the ovary tissues using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB14, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120
Immunohistochemical staining of paraffin-embedded human normal lung and lung Adenocarcinoma tissues using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB14, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3mi
MDCK cells were treated either with 30mM NaCl as negative control (left) or with GSK-3beta kinase inhibor 30mM LiCl (right) for 48 hrs. Anti-beta-catenin monoclonal antibody (clone UMAB14) was used for immunofluorescent staining.
Flow cytometric Analysis of Hela cells, using anti-CTNNB1 antibody(UM500014),(Red), compared to a nonspecific negative control antibody,(Blue).
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-beta-catenin mouse monoclonal antibody (Clone UMAB14). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-beta-catenin (Clone UMAB14) very specifically recognizes beta-catenin antigen on OriGene protein microarray chip.