Anti-CTNNB1 Antibody, Clone 9C1

Also for CTNNB1 (NM_001904)

OriGene Data

Immunogen	Full length human recombinant protein of human CTNNB1(NP_001895) produced in HEK293T cell.
Clone Name	Clone 9C1	Isotype	IgG2b
Species Reactivity	Human , Dog , Rat , Monkey , Mouse	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF	Suggested Dilutions	WB 1:200~2000, IHC 1:150, IF 1:100,
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 1
Alternative Name	armadillo; CTNNB; MRD19
Database Link	NP_001895
Function	The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Oct 2009].
Related Pathway	Adherens junction Adipogenesis Focal Adhesion Melanogenesis TGF Beta Signaling Pathway Wnt Signaling Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTNNB1 (RC208947, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTNNB1.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CTNNB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-CTNNB1 mouse monoclonal antibody. (TA502408)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CTNNB1 mouse monoclonal antibody. (TA502408)

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-CTNNB1 mouse monoclonal antibody. (TA502408)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CTNNB1 mouse monoclonal antibody. (TA502408)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody. (TA502408)

IF Image

Anti-CTNNB1 mouse monoclonal antibody (TA502408) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTNNB1(RC208947).

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
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DyLight® 594
DyLight® 633

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Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-CTNNB1 TrueMAB Antibody Clone 9C1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data