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Anti-CSTA Antibody EPR6941
Also for CSTA (NM_005213)
|A synthetic peptide corresponding to residues on the C-terminus in human Cystatin A was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; FC: 1:10 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens cystatin A (stefin A) (CSTA)|
|AREI; STF1; STFA|
|The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. Cystatin A is a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. It is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer (1).|
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Western blot - Anti-Cystatin A antibody [EPR6941]; All lanes : Anti-Cystatin A antibody [EPR6941] at 1/1000 dilution.Lane 1 : NIH 3T3 cell lysate.Lane 2 : A549 cell lysate.Lane 3 : A431 cell lysate.Lane 4 : K562 cell lysate.Lane 5 : Fetal brain tissue lysate.Lane 6 : Fetal heart tissue lysate.Lane 7 : Fetal kidney tissue lysate.Lane 8 : Human spleen tissue lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 11 kDa.Observed band size : 13 kDa .
Flow Cytometry - Anti-Cystatin A antibody [EPR6941]; ab128948 at a 1/10 dilution staining Cystatin A in permeabilized NIH 3T3 cells by flow cytometry (red), with Rabbit IgG control (green).