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Anti-CSNK2B Antibody EP1995Y


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against Casein Kinase II (beta subunit) (clone EP1995Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400525) , 20ug Explanation
100ul 325 3-7 Days
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminal of human CKII beta protein was used as an immunogen.
Clone NameEP1995Y IsotypeIgG
Species ReactivityMouse, Rat, Horse, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsFC: 1:20, ICC: 1:100, IHC: 1:100, WB: 1:500 - 2000,
Predicted MW Explanation 25 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens casein kinase 2, beta polypeptide (CSNK2B), transcript variant 1
Alternative NameCK2B; CK2N; CSK2B; G5A
Database LinkNP_001311
Entrez Gene 1460 Human
Entrez Gene 13001 Mouse
Entrez Gene 81650 Rat
FunctionCasein Kinase II (CKII, CK2, phosvitin kinase, troponin T kinase, casein kinase G) is a ubiquitous serine/threonine protein kinase that is highly conserved and commonly found in eukaryotic cells (found in the cytoplasm and the nucleus). CKII is a heterotetramer that consists of two catalytic subunits (alpha, alpha-prime) and two regulatory beta subunits (1). CKII can utilize both GTP and ATP as the phosphate donor and phosphorylates threonine and serine residues. Therefore, CKII is a multifunctional protein kinase with broad range of substrates (e.g. c-Myc, c-Myb, c-Fos, c-Jun, p53, Rb, HPVE7) and it has been implicated in various cell functions/processes from cell transformation to mitosis. (2)
Related PathwayDruggable Genome Wnt signaling pathwayAdherens junctionTight junction

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Casein Kinase 2 beta antibody [EP1995Y]; Anti-Casein Kinase 2 beta antibody [EP1995Y] at 1/1000 dilution + K562 cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 25 kDa.Observed band size : 25 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Casein Kinase 2 beta antibody [EP1995Y]; Immunohistochemical analysis of Casein Kinase 2 beta in paraffin embedded human ovary carcinoma tissue using TA307548 at a 1/100 dilution.
IF Image
Immunocytochemistry - Casein Kinase 2 beta antibody [EP1995Y]; Immunofluorescent staining of Casein Kinase 2 beta in HeLa cells using TA307548 at a 1/100 dilution.
FC Image
Flow Cytometry-Anti-Casein Kinase 2 beta antibody [EP1995Y](TA307548); Overlay histogram showing HeLa cells stained with TA307548 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.


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