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Anti-CSF2 Antibody

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SKU Description Amount Price Availability*  
TA319314
  • Rabbit polyclonal anti-GM-CSF antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400257) , 20ug Explanation
100ug 325 In Stock
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WB(1)

OriGene Data

ImmunogenThis purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human GM-CSF protein.
Clone Name IsotypeIgG
Species Reactivityhuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Granulocyte Macrophage Colony Stimulating Factor (also known as GM-CSF, Colony-stimulating factor; CSF, sargramostim and molgramostin) is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors show significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using anti-Human GM-CSF antibody shows detection of a band ~15 kDa in size corresponding to recombinant human GM-CSF (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight™649 conjugated anti-Rabbit IgG (p/n 611-143-122) secondary antibody diluted 1:20,000 in blocking buffer (p/n MB-070). Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).

 

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