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Home Antibody All anti-CRYAA antibodies

Anti-CRYAA TRUEMAB Antibody Clone OTI3B12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA505577 CRYAA (Alpha A Crystallin) mouse monoclonal antibody, clone OTI3B12 (formerly 3B12) 100ul $325 In Stock
LC424743 CRYAA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF505577 Carrier-free (BSA/glycerol-free) CRYAA mouse monoclonal antibody, clone OTI3B12 (formerly 3B12) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(1)
IHC(7)
IF(1)
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Also for CRYAA (NM_000394)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human CRYAA(NP_000385) produced in HEK293T cell.
Clone NameClone OTI3B12 IsotypeIgG2b
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF Suggested Dilutions WB 1:2000, IHC 1:150, IF 1:100,
Predicted MW Explanation 19.7 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens crystallin, alpha A (CRYAA)
Alternative NameCRYA1; CTRCT9; HSPB4
Database LinkNP_000385
Entrez Gene 1409 Human
FunctionCrystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAA (RC216946, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAA.
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CRYAA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505577)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CRYAA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505577)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CRYAA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505577)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CRYAA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505577)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CRYAA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505577)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CRYAA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505577)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CRYAA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505577)
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Anti-CRYAA mouse monoclonal antibody (TA505577) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAA(RC216946).
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