Home Antibody All anti-CRK antibodies
|The antiserum was produced against synthesized non-phosphopeptide derived from human CrkII around the phosphorylation site of tyrosine 221(G-P-YP-A-Q).|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:5000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|CrkII (Ab-221) antibody detects endogenous levels of total CrkII protein.
|Homo sapiens v-crk avian sarcoma virus CT10 oncogene homolog (CRK), transcript variant II|
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot analysis of extracts from COS7 cells, using CrkII (Ab-221) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using CrkII (Ab-221) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HuvEc cells, using CrkII (Ab-221) antibody.The picture on the right is treated with the synthesized peptide.