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Anti-CRK PHOSPHO Antibody EPR269Y
|A phospho specific peptide corresponding to regions surrounding Tyrosine 221 was used as an immunogen. This antibody detects proteins phosphorylated at Y221.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:2000; ICC: 1:50 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens v-crk avian sarcoma virus CT10 oncogene homolog (CRK), transcript variant I|
|Crk (p38) are adaptor proteins, composed mostly of SH2 and SH3 domains, which play a key role in cellular signaling by mediating the construction of protein complexes and also by managing cell proliferation, differentiation, and migration (1). They are critical integrators of upstream signals for cell invasion and migration in human cancer cell lines. Crk is believed to be a regulator of invasive responses because increased levels of the protein have been observed in multiple human cancers (2). In vivo studies have demonstrated that decreased levels of Crk remarkably inhibits tumor formation and its invasive growth (3). Crk associates with ERM family proteins (including ezrin, radixin, and moesin), activates RhoA, and promotes cell motility toward hyaluronic acid (4). |
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - Crk p38 (phospho Y221) antibody [EPR269Y]; All lanes : Anti-Crk p38 (phospho Y221) antibody [EPR269Y] at 1/2000 dilution.Lane 1 : K562 cell lysate.Lane 2 : K562 cell lysate treated with AP.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 34 kDa.Observed band size : 34 kDa.