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Home Antibody All anti-CREB1 antibodies

Anti-CREB1 PHOSPHO Antibody

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SKU Description Amount Price Availability*  
TA319433 Rabbit polyclonal CREB phospho S133 antibody (Phospho-specific) 100ul $325 3-7 Days
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WB(2)
IHC(1)
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Also for CREB1 (NM_004379)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenCREB phospho peptide corresponding to amino acid residues 122-147 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, rat Concentration0.064 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:1,000 - 1:6,000, WB: 1:500 - 1:2,000, IHC: 20 ug/ml
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMP-dependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II.  Alternate splicing of this gene results in two transcript variants encoding different isoforms.

Reference Data

Target NameHomo sapiens cAMP responsive element binding protein 1 (CREB1), transcript variant A
Alternative NameCREB
Database LinkNP_004370
Entrez Gene 1385 Human
Entrez Gene 12912 Mouse
Entrez Gene 81646 Rat
Function
Related PathwayTranscription FactorsDruggable Genome Antigen processing and presentationMelanogenesisHuntington's diseaseProstate cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Anti-CREB pS133 was used to detect phosphorylated CREB by WB. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. WB of control (-) and in-vitro phosphorylated CREB (+) was used to show that the antibody reacts specifically with the phosphorylated form. Detection occurs using a 1:500 dilution of antibody followed by 1:5,000 dilution of HRP Goat-a-Rabbit IgG with visualization via ECL.
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Anti-CREB pS133 was used to detect phosphorylated CREB by WB. Recombinant His-tagged human CREB was produced in E.coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. WB of indicated amounts of control (-) and in-vitro phosphorylated CREB (P) were loaded to show that the antibody reacts specifically with the phosphorylated form. Detection occurs using a 1:500 dilution of antibody.
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affinity purified anti-CREB pS133 antibody was used at 20 ?g/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate to strong nuclear staining of tonsillar lymphocytes. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
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