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Anti-CREB1 Antibody E306


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal Antibody against CREB1 (Clone E306)
100ul $325 3-7 Days
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues before DNA binding motif of human CREB was used as immunogen. This antibody can also recognize the splice isoform of CREB. Predicted to cross-react with bovine, based on sequence homology.
Clone NameE306 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:500 - 1:1000; ICC/IF: 1:250 - 1:500; IHC-P: Use at an assay dependent dilution; FC: 1:40; IP: 1:80
Predicted MW Explanation 37 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Reference Data

Target NameHomo sapiens cAMP responsive element binding protein 1 (CREB1), transcript variant B
Alternative NameCREB
Database LinkNP_604391
Entrez Gene 1385 Human
Entrez Gene 12912 Mouse
Entrez Gene 81646 Rat
FunctionCREB is a transcription factor protein that binds the cAMP response element (CRE), thereby stimulating transcription of this sequence. CREB plays a role in propagating numerous extracellular signals to the nuclues (1-3). CREB is activated by phosphorylation at the Ser133 position by various signaling pathways, and some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (1-3).
Related PathwayTranscription FactorsDruggable Genome Antigen processing and presentationMelanogenesisHuntington's diseaseProstate cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - CREB antibody [E306]; Anti-CREB antibody [E306] at 1/1000 dilution + A431 cell lysate.Predicted band size : 37 kDa.Observed band size : 40 kDa .
Immunohistochemistry (Paraffin-embedded sections) - CREB antibody [E306]; Ab32515, at a 1/500 dilution, staining CREB in paraffin embedded prostatic carcinoma tissue sections by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - CREB antibody [E306]; Ab32515, at a 1/500 dilution, staining CREB in PC12 cells by Immunofluorescence.
Flow Cytometry - CREB antibody [E306]; Overlay histogram showing HEK293 cells stained with TA300440 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.


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