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Home Antibody All anti-CREB1 antibodies

Anti-CREB1 Antibody E113

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300374
  • Rabbit Monoclonal Antibody against CREB1 (Clone E113)
100ul 325 3-7 Days
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WB(3)
IHC(2)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser133 of human CREB was used as immunogen. The antibody only detects CREB phosphorylated on Serine 133. Predicted to cross-react with bovine, chicken, and zebra fish, based on sequence hom
Clone NameE113 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:500; IHC-P: 1:250; FC: 1:1000; IP: 1:40; ICC/IF: 1:250
Predicted MW Explanation 37 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens cAMP responsive element binding protein 1 (CREB1), transcript variant B
Alternative NameCREB
Database LinkNP_604391
Entrez Gene 1385 Human
Entrez Gene 12912 Mouse
Entrez Gene 81646 Rat
FunctionCREB is a transcription factor protein that binds the cAMP response element (CRE), thereby stimulating transcription of this sequence. CREB plays a role in propagating numerous extracellular signals to the nuclues (1-3). CREB is activated by phosphorylation at the Ser133 position by various signaling pathways, and some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (1-3).
Related PathwayTranscription FactorsDruggable Genome Antigen processing and presentationMelanogenesisHuntington's diseaseProstate cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - CREB (phospho S133) antibody [E113]; All lanes : Anti-CREB (phospho S133) antibody [E113] at 1/500 dilution.Lane 1 : A431 cells untreated.Lane 2 : A431 cells treated with forskolin.Predicted band size : 37 kDa.Observed band size : 46 kDa .
WB Image
Western blot - Anti-CREB (phospho S133) antibody [E113]; .developed using the ECL technique.Performed under reducing conditions.Predicted band size : 37 kDa.
WB Image
Western blot - Anti-CREB (phospho S133) antibody [E113]; .Predicted band size : 37 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CREB (phospho S133) antibody [E113]; Ab32096, at a 1/250 dilution, staining CREB in paraffin embedded human thyroid gland adenocarcinoma tissue by Immunohistochemistry. .Image A: Cells are untreated. .Image B: Cells are treated with Phosphatase.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CREB (phospho S133) antibody [E113]; TA300374 staining CREB in Rat hippocampus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Sections were formalin-fixed, and subjected to antigen retrieval by autoclave prior to blocking with 8% milk for 30 minutes at 37°C. The primary antibody was diluted 1/100 with DAKO antibody diluent and incubated with the sample for 18 hours at 4°C. An LSAB-labeled Streptavidin-Biotin conjugated Goat polyclonal antibody was used undiluted as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - CREB (phospho S133) antibody [E113]; Ab32096, at a 1/250 dilution, staining CREB in A431 cells by Immunofluorescence. .Image A: Cells are untreated. .Image B: Cells are treated with Phosphatase.
FC Image
Flow Cytometry - Anti-CREB (phospho S133) antibody [E113]; Overlay histogram showing A431 cells stained with TA300374 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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