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Anti-COPS3 Antibody EPR3127
Also for COPS3 (NM_003653)
|A synthetic peptide corresponding to residues at the C-terminus of human COPS3 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB: 1:10000 - 1:20000; IP: 1:40; FC: 1:20 - 1:100; ICC/IF: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-P.
|Homo sapiens COP9 signalosome subunit 3 (COPS3), transcript variant 1|
|COPS3 is a component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. Individual subunits of the complex have been linked to various signal transduction pathways leading to gene expression and cell cycle control (1, 2). Subunit 3 (COPS3) possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-B alpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation (3). COPS3 is suggested as a specific negative regulator of TNF-induced NF-kappaB activation pathways because its over-expression inhibits NF-kappaB activation (4). |
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Western blot - COPS3 antibody [EPR3127]; All lanes : Anti-COPS3 antibody [EPR3127] at 1/20000 dilution.Lane 1 : HT-29 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : SKBR-3 cell lysate.Lane 4 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 48 kDa.Observed band size : 48 kDa.
Immunocytochemistry/ Immunofluorescence - COPS3 antibody [EPR3127]; Immunofluorescent staining of HeLa cells using ab79698 at 1/250 dilution.
Flow Cytometry-Anti-COPS3 antibody [EPR3127](ab79698); Overlay histogram showing HeLa cells stained with ab79698 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.